Human INSIG2 ELISA Kit (HUFI03805)

The Human INSIG2 ELISA Kit from Assay Genie quantifies Insulin-Induced Gene 2 protein, a regulator of cholesterol and lipid biosynthesis that anchors SREBP transcription factors within the endoplasmic reticulum. Researchers studying metabolic disease, hepatic steatosis, and sterol feedback inhibition correlate circulating INSIG2 concentrations with patient lipid profiles and treatment outcomes.

The microplate is pre-coated with monoclonal capture antibodies specific for the human antigen. A standard curve is generated using reconstituted calibrators dissolved in 1.0 mL of sample diluent, and bound analyte is detected with an HRP-conjugated secondary reagent. TMB substrate produces a measurable color change read on a standard plate reader.

Unopened kits are shipped refrigerated and should be stored at 4 °C until first use; reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles. Intended for research use only, not for diagnostic procedures.