Human Inorganic pyrophosphatase (PPA1) ELISA Kit

This sandwich-format ELISA kit from Assay Genie enables quantitative measurement of human Inorganic pyrophosphatase (PPA1) in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. Detection is colorimetric, with absorbance read at 450 nm, and the assay is specific for natural and recombinant human Inorganic pyrophosphatase.

Reported performance figures include a detection range of 15.6-1000 pg/mL and a sensitivity of 7.2 pg/mL. Precision values are 5.3% intra-assay CV and 7.2% inter-assay CV. Linearity has been demonstrated in serum (81-117%), EDTA plasma (98-118%) and heparin plasma (81-119%) across serial dilutions, with recovery averaging 84% in serum (range 80-90%) and 86% in plasma (range 80-92%). PPA1 is a cytoplasmic homodimer that catalyses the hydrolysis of pyrophosphate to inorganic phosphate, supporting cellular phosphate and energy metabolism. For research use only.