Human ING1 ELISA Kit (HUFI07771)

The ING1 protein (Inhibitor of Growth 1) is a type II tumor suppressor that regulates cell cycle progression, apoptosis, and chromatin remodeling through the p53 acetylation pathway. Researchers use this Assay Genie immunoassay to quantify endogenous ING1 in clinical and experimental samples, supporting studies in oncology, cellular senescence, and stress response signaling.

Typical applications include comparing ING1 expression between tumor and matched normal tissue, evaluating its downregulation in cancer cell lines, and exploring functional links between ING1 status and p53-mediated chemotherapeutic response. The assay workflow involves preparing standards and samples, loading them onto the precoated microplate, incubating with the biotinylated detection antibody and streptavidin-HRP conjugate, adding substrate solution, and reading absorbance to interpolate concentrations from a standard curve.

Reagents should be stored according to the product insert; allow all components to reach room temperature before use and avoid repeated freeze-thaw cycles of prepared working solutions. Each kit contains the consumables required for the stated number of determinations and is intended for research use only, not for diagnostic procedures.