Human IL5 PharmaGenie ELISA Kit

Designed by Assay Genie for immunology research in the pharmaceutical and biotech sectors, this sandwich ELISA kit enables the detection of human IL-5 in serum, plasma and cell culture supernatant samples. The assay uses a highly optimised monoclonal antibody pair paired with a purified recombinant protein standard, and the capture plates are pre-coated to support a fast protocol for target validation and analysis.

Key performance specifications quoted from the manufacturer include an assay range of 7.8 pg/ml - 250 pg/ml and a sensitivity of 5 pg/ml. The antibody pair recognises both natural and recombinant human IL-5, with no cross reactivity with other human cytokines reported. Standard curves are calibrated to NIBSC, and all reagents have been validated according to ISO 9001:2000 quality systems. Each kit includes pre-coated strip plates, a biotinylated secondary antibody, standards, controls where applicable, buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure. A 100 µl sample volume is required, with a total incubation time of 3h45, and detection is read colorimetrically.