Human IL4 SuperSet ELISA Kit

Assay Genie's DIY ELISA Kit is designed for the detection of IL-4 in serum, plasma, and cell culture supernatant samples using a colorimetric method. Reactive with human samples, the assay recognises both natural and recombinant human IL-4 and shows no cross reactivity with other human cytokines.

Quantitation is supported by an assay range of 1.1 pg/ml to 35 pg/ml and a sensitivity of 0.5 pg/ml, requiring a sample size of 100 µl, with the full workflow from sample to end completed in 3h45. Kit contents include capture and biotinylated detection antibodies, one standard per plate, Streptavidin-HRP, TMB, and a detailed procedure including buffer composition.

Developed with pharmaceutical and biotech research applications in mind, the kit provides a highly optimised antibody pair along with a purified recombinant protein for analyte detection in cell culture supernatants and is also suitable for use in more complex matrices such as serum and plasma, positioning it as a key tool for immunology research.