Human IL27 PharmaGenie ELISA Kit

A colorimetric Sandwich ELISA kit from Assay Genie designed for the detection of human IL-27 in a range of sample types. The PharmaGenie range is intended for the pharmaceutical and biotech research sectors, focusing on high-quality antibodies, purified standards, buffers and pre-coated plates as key tools for immunology research.

Each kit contains a highly optimised antibody pair together with a purified recombinant protein for analyte detection in cell culture supernatants, and is also suited to more complex matrices such as serum and plasma. Key features include a highly optimised monoclonal antibody pair for specific and sensitive analyte detection, a high-quality purified recombinant protein for consistent standard curves, validation of all reagents to ISO 9001:2000 quality systems, recognition of both natural and recombinant antigen, no cross-reactivity with other cytokines tested, standard calibration to NIBSC, and pre-coated ELISA plates with a fast protocol to support target validation and analysis.

The assay has an assay range of 31.25 pg/ml - 1000 pg/ml and a sensitivity of 12.8 pg/ml. The incubation time is 3h45 and the required sample size is 100 µl. Acceptable sample types include serum, plasma and cell culture supernatant. Kit contents comprise pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.