Human IL17F PharmaGenie ELISA Kit

The Human IL17F PharmaGenie ELISA Kit from Assay Genie is a sandwich immunoassay built on a pre-coated 96-well microplate, supplied here as two plates (2 x 96 wells) for higher throughput. The assay quantitatively measures human interleukin-17F, a pro-inflammatory cytokine secreted predominantly by Th17 cells and group 3 innate lymphoid cells, in serum and cell culture supernatants. Detection is colorimetric, typically read at 450 nm against a reference wavelength, with quantitation against a recombinant IL-17F standard curve.

Each kit ships with the pre-coated plate, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized standards, wash buffer, and TMB substrate; the workflow involves sample incubation, washing, detection antibody binding, and a chromogenic read after stop solution. Typical assay time is roughly 3-4 hours performed at room temperature. Store unopened components at 2-8 °C; reconstituted standards should be aliquoted and frozen to preserve activity across repeated freeze-thaw cycles. Intended for in vitro research use only, this kit supports cytokine profiling studies in autoimmune inflammation, mucosal host defense, psoriasis, rheumatoid arthritis, and asthma-related research.

Built around a sandwich immunoassay architecture, the Human IL-17F PharmaGenie ELISA Kit from Assay Genie delivers quantitative cytokine measurements with a standard curve range of approximately 15.6-1000 pg/mL and a sensitivity near 2 pg/mL. The capture antibody recognises the IL-17F homodimer as well as IL-17A/F heterodimers, an important consideration when interpreting data from activated Th17 cell cultures where both cytokines are co-expressed. The assay uses a 100 µL sample volume per well, a 2-hour primary incubation at room temperature, and a chromogenic TMB read at 450 nm. Validated matrices include serum and cell culture supernatant, and the kit is commonly used by researchers studying psoriasis lesions, rheumatoid arthritis synovial fluid, asthma sputum, and inflammatory bowel disease biopsy cultures. Th17 cells and group 3 innate lymphoid cells are the principal biological sources of IL-17F, and the cytokine is increasingly reported in mucosal host defense models. For long-term sample integrity, store serum at -80 °C and aliquot standards to avoid repeated freeze-thaw cycles. The pre-coated 96-well microplate, biotinylated detection antibody, and streptavidin-HRP format together support straightforward plate-based workflows in standard immunology and inflammation laboratories.