Human IL-7 CLIA Kit (HUES00428)

Researchers studying immune reconstitution and T-cell homeostasis often rely on IL-7 measurement to track thymic output and lymphocyte survival signaling. This chemiluminescence-based assay is commonly deployed in studies of HIV immunology, where declining IL-7 levels correlate with CD4+ T-cell recovery, and in tumor microenvironment research investigating cytokine support of infiltrating lymphocytes. The microplate workflow typically requires 50-100 µL of sample per well, with standard curves spanning roughly 1-1000 pg/mL to accommodate both healthy baseline values and elevated inflammatory states. Assay Genie provides lot-tested reagents with reported inter-assay and intra-assay precision profiles, supporting reproducible quantification across independent runs. The 96-well plate layout supports batch processing of patient cohorts, while compatibility with standard luminometer readers removes the need for dedicated instrumentation. For best results, aliquot reconstituted standards to limit freeze-thaw events and store the sealed plate at 2-8 °C until first use.