Human IL-6 PharmaGenie ELISA Kit (SBRS0784)

The Human IL-6 PharmaGenie ELISA Kit from Assay Genie enables quantitative measurement of interleukin-6 (IL-6), a key pro-inflammatory cytokine implicated in acute-phase response, immune regulation, autoimmune disease, cancer progression, and cytokine release syndrome. Researchers use this kit to profile inflammatory biomarkers in clinical research samples, validate therapeutic candidates, and monitor immune cell activation in cell culture and animal models.

Assay Performance:

• Detection range: 15.6 to 1000 pg/mL
• Sensitivity: less than 5 pg/mL
• Sample volume: 100 µL per well
• Validated for cell culture supernatants, cell lysates, plasma, serum, and tissue lysates
• Human-specific capture antibody with no significant cross-reactivity with rodent IL-6

Kit Contents and Handling: Each kit includes a pre-coated microplate, lyophilized recombinant IL-6 standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate, and stop solution. Unopened kits are shipped refrigerated and stored at 4°C; reconstituted standards and detection antibody should be aliquoted and frozen at -20°C to limit freeze-thaw cycles, with reagents protected from light when not in use. The recommended workflow follows an ELISA protocol with incubation, washing, and absorbance readout on a compatible plate reader.

For research use only.