Human IL-5RA ELISA Kit

A sandwich ELISA from Assay Genie for the quantitative measurement of human Interleukin-5 receptor subunit alpha (IL-5RA), also known by the synonyms CDw125, CD125, and IL5R. The kit is reactive with human samples and is intended for research use only within the field of immunology.

The assay accepts serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Reported performance includes a detection range of 78-5000 pg/mL, a sensitivity of 32 pg/mL, an intra-assay CV of 6.4%, and an inter-assay CV of 9.6%. Recovery is 98% (range 92-104%) in serum and 100% (range 94-106%) in plasma. Specificity is stated for natural and recombinant human IL-5RA, and the colorimetric readout is measured at 450 nm.

IL-5RA is a single-pass type I membrane protein that forms the alpha subunit of the interleukin-5 receptor and directly binds IL-5. It heterodimerises with a beta subunit shared by the receptors for IL-3, IL-5, and GM-CSF.