Human IL-5 PharmaGenie ELISA Kit (SBRS0781)

The Human IL-5 PharmaGenie ELISA Kit from Assay Genie is intended for quantitative measurement of interleukin-5, a Th2-associated cytokine that drives eosinophil proliferation, activation, and recruitment in allergic airway disease, helminth infection, and B-cell maturation studies. Researchers typically load 100 uL of standard or diluted sample per well, with cell culture supernatant, EDTA-plasma, and clotted-serum input types validated by the manufacturer.

Each kit contains a capture-antibody-coated plate, lyophilised recombinant human IL-5 standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, assay diluent, TMB substrate, and stop solution, with sufficient reagents for one full plate run. Unopened kits should be stored at -20 degrees C in a frost-free freezer; reconstituted standards and working detection antibody remain stable for short-term use when kept refrigerated at 4 degrees C, and standard aliquots are recommended to limit freeze-thaw cycles. The product is labelled for research use only and is widely cited in asthma pathogenesis, eosinophilic oesophagitis, hypereosinophilic syndrome, and vaccine-adjuvant immunology studies.