Human IL-5 ELISpot Pair

This matched antibody pair from Assay Genie is intended for quantitative ELISpot enumeration of human IL-5-secreting cells at single-cell resolution. Interleukin-5 is a Th2-lineage cytokine that supports eosinophil maturation and activation, making this pair relevant to allergy, asthma, helminth immunity, and eosinophilic disorder research.

The capture and biotinylated detection antibodies are supplied as a pair for setup on PVDF-membrane or nitrocellulose-backed microplates, where IL-5 spot-forming cells (SFCs) can be counted following polyclonal or antigen-specific stimulation of peripheral blood mononuclear cells (PBMCs). The kit is configured for research use only; store refrigerated and protect frozen aliquots from repeated freeze-thaw cycles. Human-specific reactivity has been confirmed, with no significant cross-reactivity observed against the corresponding murine cytokine, supporting clear discrimination in xenogeneic or comparative immunology studies.