Human IL-5 ELISpot Kit

This ELISpot kit from Assay Genie enables single-cell resolution detection of IL-5 secreting cells, supporting immunology and vaccine research workflows. IL-5 is a Th2-associated cytokine central to eosinophil biology, and the assay is widely used to enumerate antigen-specific responses in PBMC populations following stimulation. The kit is provided as a complete ready-to-use set containing a pre-coated microplate, biotinylated detection antibodies, streptavidin-enzyme conjugate, substrate solution, and wash buffer, requiring only standard laboratory equipment and a cell culture incubator at 37 °C with 5% CO₂.

Typical applications include monitoring cytokine responses in allergy and asthma research, infectious disease studies, and immune monitoring of vaccine candidates. Researchers commonly use the assay with human peripheral blood mononuclear cells cultured overnight in vitro, then count spot-forming cells using an automated reader or stereomicroscope. Store all reagents refrigerated at 4 °C, protect the substrate from light, and avoid repeated freeze-thaw cycles of the antibody components to preserve activity across the full shelf life of the kit.