Human IL-4 ELISpot Pair

This ELISpot Pair from Assay Genie provides matched capture and detection antibodies for building a single-cell cytokine secretion assay against human IL-4, a Th2-associated cytokine central to B-cell class switching and IgE regulation. The pair uses a human-specific capture reagent, enabling clear discrimination of IL-4-producing cells from stimulated peripheral blood mononuclear cells or splenocytes, with each secreting cell visualized as a discrete spot via a colorimetric substrate on a membrane-backed plate. The flexible pair format supports custom plate layouts and works with cell culture supernatants and serum, fitting vaccine, allergy, and infectious-disease immunology workflows. Store reagents refrigerated; protect from repeated freeze-thaw cycles. For research use only.