Human IL-28B/IFN-lambda 3 PharmaGenie ELISA Kit (SBRS0768)

Detect and quantify interleukin-28B (IFN-lambda 3), a type III interferon central to antiviral mucosal immunity and hepatic inflammation, in human biological samples. This Sandwich ELISA from Assay Genie uses a pre-coated microplate with capture antibodies specific to IL-28B, paired with biotinylated detection antibody and streptavidin-HRP conjugate. Each kit provides reagents for one 96-well plate, with a standard curve generated from serially diluted recombinant human IFN-lambda 3. Plates are read at 450 nm with a 570 nm reference correction, and the full protocol finishes in approximately 4.5 hours from sample addition to final absorbance. Capture antibody pairs are validated for human IFN-lambda 3 with no significant cross-reactivity against related type III interferons IL-28A and IL-29, supporting specific quantification in immunology, hepatology, and infectious disease research. Reagents should be stored refrigerated at 2-8°C; reconstituted standards and biotinylated antibody aliquots remain stable through multiple freeze-thaw cycles when handled as directed.