Human IL-28 R alpha PharmaGenie ELISA Kit (SBRS0769)

IFNLR1 (also called IL-28 receptor alpha) is the ligand-binding subunit of the heterodimeric receptor for type III interferons, namely IFN-λ1/IL-29, IFN-λ2/IL-28A, and IFN-λ3/IL-28B. Engagement of IFNLR1 with its partner chain IL-10Rβ triggers JAK1/TYK2 activation and downstream STAT1/STAT2 phosphorylation, producing an antiviral transcriptional program that is functionally distinct from classical type I IFN signaling through IFNAR. Because IFNLR1 expression is enriched on mucosal epithelial cells, hepatocytes, plasmacytoid dendritic cells, and select B-cell subsets, soluble receptor quantification using this Assay Genie ELISA supports investigations of HCV/HBV pathogenesis, SARS-CoV-2 and influenza responses, and inflammatory bowel disease models. For biofluid workup, permit whole blood to clot for 30 minutes at room temperature before centrifugation at 1000 x g for 15 minutes; aliquot the clarified supernatant and store at -20 °C, limiting freeze-thaw cycles to preserve epitope integrity. Supernatants from stimulated epithelial monolayers may be applied neat or diluted in assay diluent if expected values exceed the upper 10 ng/mL calibrator. Researchers comparing IFNLR1 levels against IFNAR2, IL-10Rβ, or downstream ISG readouts (MX1, OAS1, IFIT1) will find the standard curve linear across the working range and broadly compatible with parallel ELISA panels from the same Assay Genie catalog.