Human IL-27 ELISA Kit (HUEB0064)

A Sandwich ELISA Kit from Assay Genie is configured for the quantitative detection of human IL-27 (Interleukin-27 subunit alpha, also known as Interleukin-30, p28, IL27A, IL30), using a colorimetric detection method. The assay is reactive with human samples and is supplied for research use only.

Reported performance characteristics include a detection range of 15.6-1000pg/mL, a sensitivity of 3.9pg/mL, an intra-assay CV of 4.2% and an inter-assay CV of 7.8%. Specificity is directed against natural and recombinant human Interleukin-27 subunit alpha.

Validated sample matrices include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The target is a secreted heterodimer formed with EBI3 (UniProt Q8NEV9), a member of the IL-6 superfamily with a reported molecular weight of 27,493 Da, which functions in innate immunity and in the regulation of T-helper cell development.