Human IL-2 ELISpot Pair

The Human IL-2 ELISpot Pair from Assay Genie provides matched capture and biotinylated detection antibodies for quantitative measurement of Interleukin-2 (IL-2) secretion at the single-cell level. The antibody pair enables coating of PVDF-bottomed microplates, supporting enumeration of IL-2-producing cells following antigen-specific or polyclonal stimulation of peripheral blood mononuclear cells. The workflow accommodates cell culture supernatant transfer after overnight incubation, with a 100 uL sample volume per well yielding reproducible spot formation. Researchers apply this reagent in T cell function studies, vaccine development, and immune monitoring workflows. Each pair ships as a 96 Assays format kit intended for research use only (RUO).