Human IL-2 ELISA Kit (HUEB0025)

From Assay Genie, this Sandwich ELISA is configured for the quantitative detection of human Interleukin-2 (IL-2) using a colorimetric read-out. The assay is reactive with human samples and has been validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity toward natural and recombinant human Interleukin-2.

Performance characteristics reported by the manufacturer include a detection range of 15.6-1000pg/mL, a sensitivity of 10pg/mL, an intra-assay CV of 4.6% and an inter-assay CV of 7.8%. Recovery is reported as averaging 95% (range 89-101%) for serum and 97% (range 91-103%) for plasma. Linearity has been evaluated across serum, EDTA plasma and heparin plasma at 1:2, 1:4, 1:8 and 1:16 dilutions. The kit is listed for research use only and is associated with the cancer research area.

IL-2 is a secreted cytokine produced by T-cells in response to antigenic or mitogenic stimulation and is required for T-cell proliferation and other activities central to regulation of the immune response. It can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The protein is catalogued under UniProt entry P60568.