Human IL-1R1/IL-1 Receptor I ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of human IL-1R1 (Interleukin-1 Receptor Type I, UniProt P14778) in serum and plasma samples, with a colorimetric readout.

The workflow includes a 90-minute primary incubation at 37°C, a 60-minute biotin-labelled detection antibody step, a 30-minute HRP-Streptavidin incubation, and a 10–20 minute TMB substrate development in the dark, with absorbance read at 450 nm after the stop solution is added. Unopened components are stored at 2–8°C with a 12-month shelf life. Reported linearity for serum (n=5) is 86–102% at 1:2, 94–100% at 1:4, and 82–99% at 1:8, with EDTA plasma at 92–104%, 87–98%, and 80–93%, and heparin plasma at 85–103%, 83–93%, and 92–100% across the same dilutions. Spike-and-recovery averages are 95% in serum, 94% in EDTA plasma, and 94% in heparin plasma.

Synonyms listed for the target include IL1R1, Interleukin 1 Receptor Type I, and IL-1R1. Researchers should follow the protocol provided with the specific kit lot.