Human IL-1A ELISA Kit (HUEB0123)

Manufactured by Assay Genie, this colorimetric Sandwich ELISA Kit is designed for the quantitative detection of human Interleukin-1 alpha (IL-1A) in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The assay has a stated detection range of 15.6-1000pg/mL and a sensitivity of 5.6pg/mL, with an intra-assay CV of 5.3% and an inter-assay CV of 8.2%. Specificity is reported for natural and recombinant human Interleukin-1 alpha.

IL-1A, also known by the synonyms Hematopoietin-1, IL-1 alpha, and IL1F1, is a monomeric proinflammatory cytokine. According to the manufacturer description, it is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. The protein is identified as an endogenous pyrogen and is reported to stimulate the release of prostaglandin and collagenase from synovial cells. The associated UniProt entry is P01583, with a molecular weight of 30,607 Da. Research applications are oriented toward the neurosciences area.

Linearity has been evaluated in serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16. Recovery data report an average of 95% (range 89-101%) for serum and 97% (range 91-103%) for plasma. The kit is intended for research use only.