Human IL-18 R beta PharmaGenie ELISA Kit (SBRS0737)

The IL-18 receptor beta (IL-18RAP) PharmaGenie ELISA Kit from Assay Genie provides quantitative measurement of the accessory subunit of the heterodimeric IL-18 receptor in human research samples. IL-18RAP pairs with the ligand-binding IL-18R alpha chain to confer high-affinity IL-18 binding and to drive downstream NF-kB and MAPK signaling that culminates in IFN-gamma release from Th1 lymphocytes and NK cells. Accurate quantification of this receptor subunit therefore supports pathway-focused studies in inflammatory disease, infectious immunity, and cytokine receptor pharmacology.

Each kit is configured for 96 assays and supports work with cell culture supernatant samples as well as isolated plasma fractions and matched serum fractions from human donors. The assay follows a standard ELISA workflow with an absorbance-based read-out; all components should be stored refrigerated between uses, and reconstituted standards or detection antibody are best aliquoted to limit freeze-thaw cycles and preserve inter-assay consistency.

Typical research applications include profiling receptor expression on activated immune cells, screening small-molecule or antibody modulators of the IL-18 axis, and benchmarking patient or donor cohorts in autoimmune, inflammatory, or infectious disease research programs. The kit supplies pre-coated microplate wells, biotinylated detection reagent, standard diluent, wash buffer, substrate solution, and stop reagent sufficient for a full 96-assay plate, and is intended for research use only.