Human IL-18 PharmaGenie ELISA Kit (SBRS0735)

Assay Genie's Human IL-18 PharmaGenie ELISA Kit enables quantitative measurement of interleukin-18, a pro-inflammatory cytokine central to TH1 immune responses and IFN-gamma induction. This immunoassay supports immunology research across inflammatory disease, autoimmune conditions, infectious disease studies, and oncology biomarker investigations.

The complete protocol finishes in approximately 3.5 hours, with room temperature incubation steps and standard wash procedures between reagent additions.

Each kit ships with a strip plate pre-coated with capture antibody, biotinylated detection reagent, streptavidin-HRP conjugate, lyophilized standards, wash buffer concentrate, TMB substrate solution, and stop reagent in sealed vials. Standard curves are generated in duplicate, and results are interpolated against a four-parameter logistic fit for accurate cytokine quantification across the calibration span.

IL-18, originally characterized as interferon-gamma-inducing factor, drives natural killer cell activation and T-cell polarization, bridging innate and adaptive immunity. Circulating IL-18 concentrations inform studies of sepsis prognosis, rheumatoid arthritis severity, lupus nephritis activity, and metabolic inflammation. The Assay Genie protocol supports translational workflows, drug development screening, and clinical sample characterization without significant cross-reactivity with related IL-1 family cytokines.