Human Il-17 B PharmaGenie ELISA Kit (SBRS0728)

Beyond the protocol details already described, this Assay Genie PharmaGenie kit provides additional research-relevant features. The capture antibody is human-specific and demonstrates no significant cross-reactivity with related cytokines IL-17A, IL-17C, IL-17D, IL-17E, or IL-17F, allowing clear discrimination of the IL-17B isoform when patient specimens contain multiple IL-17 family ligands. IL-17B signals through the IL-17RB receptor rather than the IL-17RA receptor engaged by IL-17A, so this kit supports specific interrogation of the IL-17B/IL-17RB pathway in inflammatory bowel disease, rheumatoid arthritis, and tumor microenvironment studies where this signaling axis has been implicated. Liquid biospecimens such as serum specimens, plasma specimens, or harvested cell culture supernatant should be prepared according to the kit insert, with matrix samples commonly diluted in assay diluent before loading 100 µL per well. The pre-coated plate uses a two-antibody binding configuration with sequential addition of sample, biotinylated detection reagent, and streptavidin-HRP, followed by TMB substrate development for absorbance-based quantification at the kit-recommended wavelength. Following the standard curve protocol, intra-assay precision is generally maintained below 10% CV across replicate wells, supporting reliable comparison of IL-17B concentrations between patient cohorts and matched controls in translational immunology research.