Human IL-15 PharmaGenie ELISA Kit (SBRS0724)

Assay Genie's IL-15 PharmaGenie format uses a 7-point standard curve generated from the reconstituted lyophilized reference standard, with each duplicated standard well producing a linear fit suitable for interpolating unknown samples across the working range. The single-batch incubation runs approximately 3.5 hours at room temperature, after which absorbance is recorded. Spike-and-recovery experiments in serum and plasma matrices typically fall within an 85 to 115 percent range, while dilution linearity in cell culture supernatant remains above 95 percent when tested across 1:2 to 1:8 dilutions. The capture antibody pair is human-specific, showing no significant cross-reactivity with recombinant human IL-2, IL-4, IL-7, or IL-21 at concentrations up to 10 ng/mL, an important consideration when profiling cytokine networks where these gamma-chain family members are co-expressed. Reconstituted working stocks remain stable for up to 30 days at -20 °C when aliquoted to avoid repeated freeze-thaw cycles, and the kit ships in an insulated container with cold packs to preserve enzymatic conjugate activity. This ELISA is configured exclusively for research use only and is not validated for diagnostic or therapeutic decision-making.