Human IL-13 ELISpot Pair

This Assay Genie ELISpot Pair is configured to detect human interleukin-13 (IL-13) secretion at the single-cell level, enabling enumeration of cytokine-producing cells from cultured PBMCs or other immune cell populations. IL-13 is a Th2-associated cytokine central to studies of allergic inflammation, asthma pathogenesis, parasitic helminth immunity, and mucosal immune responses, making this reagent useful for vaccine development, immunomonitoring, and T-cell polarization research.

The pair format supplies matched capture and detection antibodies along with development reagents to visualize spot-forming units on PVDF-backed plates. Applied to cell culture supernatants and serum, the assay supports both qualitative identification of IL-13 producers and quantitative comparison of responder frequencies between donors or treatment cohorts. Components are intended for research use only and should be kept refrigerated between uses, with working aliquots handled to limit repeated freeze-thaw cycles and preserve antibody binding activity.