Human IL-12 (p40) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of human IL-12 (p40) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Using a double-antibody sandwich ELISA format with colorimetric detection, the kit provides a robust workflow for measuring this immune-response cytokine in research applications.

Performance specifications include a sensitivity of 18.75 pg/mL and a standard curve range of 31.25–2000 pg/mL. Reagents should be stored at 2–8°C for up to 12 months. The kit's target, IL-12 p40 (also known by the synonyms IL12B, IL-12B, CLMF2 and NKSF2; UniProt P29460), is a key regulator of pro-inflammatory cytokine production and T cell activation, with elevated levels linked to autoimmune, inflammatory and infectious diseases.

Reported linearity and recovery data across serum, EDTA plasma and heparin plasma demonstrate consistent performance across the recommended dilution range, supporting reliable quantification of IL-12 p40 in human biological samples.