Human IL-10 ELISpot Kit

The Human IL-10 ELISpot Kit from Assay Genie enables single-cell detection of IL-10 secreting cells in human peripheral blood mononuclear cells and lymphoid tissue cultures. This cell-based immunoassay captures cytokine release at the site of secretion, producing discrete spots that are quantified to determine the frequency of cytokine-secreting cells within a tested population.

The microplate provides pre-coated wells with capture antibody specific to human IL-10, along with biotinylated detection antibody, streptavidin-enzyme conjugate, substrate solution, and recombinant standard for spot calibration. Each well typically receives a 100 uL sample volume during the assay procedure. Researchers commonly use this ELISpot to monitor regulatory T cell function, Th1/Th2/Th17 cytokine profiling, and anti-inflammatory responses in vaccine, autoimmune, and infectious disease studies.

Key specifications include colorimetric readout, reactivity restricted to human samples, and compatibility with cell culture supernatants and serum. The assay supports research use only workflows in immunology laboratories, with sensitivity capable of resolving low-frequency cytokine-secreting populations.