Human IL-1 F6 PharmaGenie ELISA Kit (SBRS0742)

The Assay Genie Human IL-1 F6 PharmaGenie ELISA Kit enables quantitative measurement of interleukin-36 alpha in human research and clinical samples, supporting studies of pro-inflammatory cytokine signaling. IL-1 F6 (also called IL-36α) signals through the IL-36 receptor to activate NF-κB and MAPK pathways, and is investigated in psoriatic skin inflammation, mucosal immune responses, and autoimmune disease models.

The kit is supplied as a lyophilized standard, pre-coated microplate, assay buffers, detection antibody, and substrate solution for generating a calibration curve. Researchers should refrigerate all components upon receipt, allow the lyophilized standard to equilibrate before reconstitution, and aliquot the reconstituted standard for frozen storage to limit freeze-thaw degradation. All reagents are intended for research use only and are not approved for diagnostic procedures.

Applications include profiling cytokine release in stimulated primary cell cultures, monitoring IL-36α concentrations in patient-derived specimens, and benchmarking novel anti-inflammatory compounds in vitro. The capture antibody is human-specific, providing reliable discrimination in experiments where rodent models serve as negative controls.