Human IL-1 alpha PharmaGenie ELISA Kit (SBRS0738)

This PharmaGenie ELISA from Assay Genie quantifies human interleukin-1 alpha (IL-1α), a key pro-inflammatory cytokine involved in fever, acute-phase response, and immune cell activation. The assay uses a sandwich immunoassay format with a capture antibody specific to human IL-1α, minimizing cross-reactivity with IL-1β or other related family members.

Working on a standard 96-well microplate layout, the kit includes pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant standards, and TMB substrate.

Each run requires 100 µL of sample per well, with a chromogenic readout measured at 450 nm after standard washing and incubation steps. Intended for in vitro research use only; store reagents refrigerated at 2-8°C and avoid freeze-thaw cycles of the standard to preserve binding activity.