Human IKZF3 ELISA Kit (HUFI03839)

From Assay Genie, this quantitative immunoassay kit measures Ikaros family zinc finger protein 3 (Aiolos), a lymphoid-restricted zinc-finger transcription factor involved in B-cell maturation, plasma-cell differentiation, and transcriptional programs altered in lupus, rheumatoid arthritis, and certain B-cell malignancies. Intended for research use only, the kit arrives as a complete 96-microplate bundle with pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-conjugated horseradish peroxidase, substrate solution, wash concentrate, and assay diluent.

The intended application is profiling of human citrated plasma and serum specimens, although the recombinant calibrator also accommodates lysate workflows from lymphocyte-derived cell lines. The antibody pair is validated against full-length human IKZF3 with no meaningful cross-reactivity against the closely related family member IKZF1 (Ikaros), supporting selective readouts in co-expression or knockout comparison studies. Translational groups studying chronic lymphocytic leukemia treatment response, regulatory T-cell signaling, and autoantibody-associated pathways have used this format for longitudinal sample cohorts.

Storage: keep unopened plates refrigerated and protect from light; reconstituted standards should be aliquoted to limit freeze-thaw cycles. Reconstituted detection antibody is stable for short-term refrigerated use as detailed in the enclosed protocol insert.