Human IGKV1-5 ELISA Kit (HUFI03835)

Quantitative immunoassay for measuring human Immunoglobulin Kappa Variable 1-5 (IGKV1-5) protein levels, manufactured by Assay Genie for research applications.

This ELISA kit targets the IGKV1-5 gene product, a variable region of the immunoglobulin kappa light chain that contributes to antibody diversity in B cells. The assay is intended for in vitro research use only and is configured for high-throughput laboratory workflows with a microplate format.

Each kit ships with pre-coated microplate wells, calibrators, detection reagent, buffers, and substrate components needed to build a standard curve and quantify IGKV1-5 in test specimens. Researchers studying B-cell receptor repertoire, antibody engineering, lymphoma biology, autoimmune disease mechanisms, and immune repertoire sequencing commonly apply this kit to measure IGKV1-5 expression.

The standard workflow involves dispensing samples and standards onto the plate, incubation, washing, addition of detection antibody, and signal development for absorbance reading. Store unopened kit components according to the product insert; equilibrate reagents to room temperature before use. The assay is intended strictly for research use and is not validated for diagnostic or therapeutic decision-making.