Human IGFBP4 ELISA Kit

From Assay Genie, this ELISA kit is designed for the precise and reliable measurement of Insulin-like Growth Factor Binding Protein 4 (IGFBP4) in human samples. It uses a Sandwich ELISA format with double antibody detection and a colorimetric readout, making it suitable for research into IGFBP4 biology, where this protein binds insulin-like growth factors and regulates their bioavailability, influencing cell growth, differentiation, and metabolism. Dysregulation of IGFBP4 has been implicated in cancer, diabetes, and cardiovascular disorders, making it a potential biomarker for disease progression and therapeutic intervention.

The kit works with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and shows reactivity with human samples. It offers a sensitivity of 1.875 ng/mL and a measurable range of 3.125–200 ng/mL. Reported linearity falls within 81–105% across serum, EDTA plasma, and heparin plasma dilutions, with recovery averaging 95–96%. The procedure follows a six-step sandwich workflow involving reagent equilibration, primary incubation, biotinylated detection antibody binding, HRP-streptavidin binding, TMB color development, and absorbance reading at 450 nm. Kit components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer, plate sealer, and technical manual, with all components stored at 2–8°C and a stated shelf life of 12 months.