Human IGFBP-4 PharmaGenie ELISA Kit (SBRS0706)

This matched-antibody capture immunoassay from Assay Genie is calibrated for quantitative detection of human insulin-like growth factor binding protein 4, supporting research into IGF axis biology, metabolic regulation, and tumor microenvironment studies.

The kit is validated for cell culture supernatant specimens as well as serum-derived and plasma-derived sample matrices. It accommodates workflows used by laboratories investigating PAPP-A mediated proteolysis, pregnancy-associated biomarker dynamics, and bone remodeling mechanisms where IGFBP-4 plays a regulatory role.

Each kit contains reagents sufficient for 96 individual determinations run on a microplate format. The procedure pairs a coated capture antibody with a biotinylated detection antibody and a streptavidin-HRP reporter, generating an absorbance signal proportional to bound analyte after addition of substrate and stop solution. Plates are read with a standard microplate absorbance reader for straightforward integration into routine laboratory immunoassay workflows.

Insulin-like growth factor binding protein 4, a ~25 kDa secreted polypeptide, modulates bioavailability of IGF-1 and IGF-2 and circulates in extracellular fluids. Altered expression has been implicated in cardiovascular research, oncology studies, and metabolic disease investigations, making reliable human-specific quantification valuable for translational research programs.