Human IGF-II/Insulin-like growth factor II ELISA Kit

Quantitative immunoassay for measuring IGF-II in human samples

This Assay Genie kit provides a quantitative in vitro method for measuring Insulin-like Growth Factor II (IGF-II) in human blood-derived samples such as serum or plasma. IGF-II is a 7.5 kDa polypeptide hormone structurally related to insulin, with established roles in fetal and placental growth, tissue differentiation, and metabolic regulation. It is widely studied in cancer biology, growth disorder research, and metabolic disease investigations.

The kit contains a pre-coated microplate, lyophilized recombinant IGF-II standards, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, and wash buffer. Following the standard protocol, researchers add 100 µL of sample per well, incubate, wash, and add the detection reagents, with the final chromogenic read-out measured on a standard microplate reader.

Typical applications include biomarker studies in oncology, comparative analysis of IGF axis components, cell culture supernatant quantification, and longitudinal sample analysis. The microplate format supports up to 96 individual determinations per kit, with standards run in duplicate for accurate curve fitting.

Reagents should be stored refrigerated at the conditions specified in the product manual, with the standard reconstituted and aliquoted into single-use vials for repeated experiments. Shelf life and stability information are provided with each lot certificate of analysis. Research use only — not for diagnostic procedures.