Human IFT27 ELISA Kit (HUFI03844)

The IFT27 protein (Intraflagellar Transport 27) is a small GTPase-like component of the IFT-B complex that drives bidirectional cargo transport along axonemal microtubules in primary and motile cilia. This quantitative immunoassay from Assay Genie is configured to measure IFT27 levels in human specimens, supporting research into ciliopathies such as Bardet-Biedl syndrome (BBS19), Joubert syndrome, short-rib thoracic dysplasia, and broader ciliogenesis pathways.

Supplied as a 96 Assays pack, the kit uses a standard curve-based readout suitable for laboratory immunology workflows and includes a pre-coated microplate, biotinylated detection reagents, recombinant human standards, and assay buffers. Kit components should be stored refrigerated and protected from light until first use, and the reconstituted reagents are typically aliquoted to limit freeze-thaw cycles. Researchers studying hedgehog signaling, photoreceptor outer segment maintenance, renal epithelial polarity, and polycystic kidney disease will find the kit useful for profiling patient-derived samples and cell culture models in research use only settings.

IFT27 has been linked to BBS through its role in stabilizing the BBSome and trafficking cargo to the ciliary tip and base, making it a target of interest for studies of non-syndromic obesity, retinal degeneration, polydactyly, and situs inversus. The assay provides a reproducible tool for investigating IFT27 expression dynamics, comparing protein abundance across genotyped patient cohorts, and validating findings from western blot or transcriptomic studies in cilia-focused laboratories.