Human IFNA6 ELISA Kit (HUFI07776)

This kit enables quantitative measurement of human interferon alpha-6 (IFNA6) protein in human blood-derived samples. IFNA6 is a type I interferon involved in antiviral defense, innate immune signaling, and modulation of adaptive immunity, making this ELISA useful for viral infection studies, autoimmune disease research, tumor immunology, and cytokine profiling in translational workflows.

The workflow uses a pre-coated microplate with an immobilized capture antibody, a biotinylated detection antibody, and streptavidin-HRP conjugate to generate a measurable HRP-substrate signal. A standard curve is generated from recombinant IFNA6 calibrator dilutions. Recommended sample volume is 100 uL per well; standards and unknowns should be run in duplicate for reliable interpolation.

Collect whole blood by standard venipuncture, separate the desired fraction by centrifugation, aliquot, and store frozen at -20 degrees C or below to limit freeze-thaw cycles. Reagent components are shipped refrigerated on cold packs and should be refrigerated upon arrival; protect the standard and biotinylated antibody from light. This Assay Genie ELISA kit is supplied as a complete 96 Assays format and is intended for in vitro research use only.