Human IFN-gamma ELISpot Pair

The Human IFN-gamma ELISpot Pair from Assay Genie provides matched capture and biotinylated detection antibodies for researchers assembling custom ELISpot assays to enumerate IFN-gamma-secreting cells at single-cell resolution. ELISpot technology captures cytokine released by individual cells as discrete spots, allowing quantification of antigen-specific T cell frequencies in PBMC samples. Applications include infectious disease research such as tuberculosis and viral immunology studies, cancer immunotherapy monitoring, vaccine efficacy testing, and characterization of cell-mediated immunity. The pair supports workflows using either PVDF or nitrocellulose plates, with detection via a streptavidin-enzyme conjugate (research use only). Aliquot the antibody components upon arrival and store frozen at -20 °C; protect from light and minimize freeze-thaw cycles to preserve activity. Provided in a 96 Assays size, the pair gives flexibility for laboratories adapting it to their existing ELISpot instrumentation and protocol.