Human IFN gamma DIY ELISA Kit

The DIY (do-it-yourself) format from Assay Genie supplies all critical reagents in bulk — capture and detection antibodies, recombinant IFN gamma standard, streptavidin-HRP conjugate, coating buffer, wash concentrate, TMB substrate, and stop solution — so the researcher coats their own microplate, choosing well density and plate count to match throughput. Interferon gamma is a 17 kDa homodimeric cytokine central to Th1-type immunity, and quantifying its release is a routine readout in T cell activation studies, vaccine potency assays, infectious disease research, and inflammation models. The absorbance-based enzymatic readout is measured on a standard microplate reader after stopping the reaction. Because IFN gamma is secreted at low pg/mL concentrations in cell culture supernatants and serum, researchers commonly load 100 uL of diluted sample per well when following the recommended protocol. Reagents should be stored refrigerated upon arrival, with the standard and conjugate handled as aliquots to limit freeze-thaw cycles and preserve activity across the shelf life of the kit.