Human IFN alpha PharmaGenie ELISA Kit (SBRS0694)

The Human IFN alpha PharmaGenie ELISA Kit from Assay Genie is a quantitative sandwich immunoassay for measuring interferon-alpha (IFN-α), a type I cytokine central to antiviral innate immunity and widely studied in viral infection research, autoimmune disease models, and vaccine adjuvant studies. The assay uses a pre-coated 96-well microplate with a biotinylated detection antibody and streptavidin-HRP for colorimetric readout, and the capture antibody specifically detects human IFN-α with no significant cross-reactivity with IFN-β or IFN-γ. The kit requires a 100 µL sample volume per well, and the plate is read at 450 nm after the final substrate incubation. Reconstituted standards should be used promptly, and unused reagents stored at 2-8°C. Researchers typically evaluate IFN-α secretion from stimulated peripheral blood mononuclear cells or from treated cell culture supernatants to characterize cytokine responses. The assay is calibrated against a recombinant IFN-α standard, producing a standard curve for interpolating unknown concentrations across cell culture supernatant, plasma, and serum samples, supporting reproducible cytokine profiling in human immunology and infectious disease research.