Human IFABP/FABP2 ELISA Kit

Designed by Assay Genie for research use, this sandwich ELISA employs a double-antibody format with colorimetric detection to quantify intestinal fatty acid-binding protein (IFABP/FABP2) in human samples. The assay supports serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, and the kit is stored at 2-8°C for 12 months.

Linearity has been validated across 1:2, 1:4, and 1:8 dilutions in serum (89-105%, 87-105%, 89-105%), EDTA plasma (88-99%, 83-96%, 83-98%), and heparin plasma (82-100%, 82-97%, 80-100%) (n=5 each). Recovery ranges from 85-104% across the same sample types, with averages of 94% for serum, 95% for EDTA plasma, and 98% for heparin plasma.

The procedure includes a 90-minute primary incubation at 37°C, a 60-minute biotin-labeled detection antibody incubation, a 30-minute HRP-Streptavidin (SABC) binding step, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution addition. The kit contains a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate SABC (100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, plate sealer, and a technical manual. IFABP is involved in intestinal fatty acid transport and lipid metabolism, and abnormal levels have been associated with inflammatory bowel disease, celiac disease, and intestinal ischemia.