Human IDNK ELISA Kit (HUFI07783)

Quantitative measurement of human IDNK protein supports research into metabolic regulation and mitochondrial biology. The IDNK gene encodes gluconokinase, a mitochondrial enzyme that phosphorylates D-gluconate to produce 6-phosphogluconate, an intermediate connecting carbohydrate catabolism to the pentose phosphate pathway. Investigators studying glucose homeostasis, NADPH balance, or metabolic reprogramming in cancer and inflammatory disease models apply this in vitro assay to quantify IDNK expression in prepared human samples.

Assay Genie supplies the kit as a complete microplate-based package containing pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and chromogenic substrate for signal development. The protocol accommodates standard curve generation in duplicate, plus dedicated wells for unknowns, allowing robust quantification across experimental conditions. Unopened kits should be refrigerated to preserve reagent activity, and all components are intended for research use only. The format supports a single microplate run with appropriate quality controls, making the procedure appropriate for translational research projects focused on metabolic enzyme biomarkers.