Human IDI2 ELISA Kit (HUFI07782)

The IDI2 enzyme, also known as isopentenyl-diphosphate delta isomerase 2, catalyzes the interconversion of isopentenyl pyrophosphate and dimethylallyl pyrophosphate within the isoprenoid biosynthesis pathway, supplying precursors for cholesterol, dolichol, and prenylated protein production. This quantitative immunoassay from Assay Genie is supplied as a complete kit of 96 Assays and is intended for research use only, enabling measurement of IDI2 protein levels in human samples.

As a peroxisomal paralog of IDI1, IDI2 supports the mevalonate-independent supply of isoprenoid building blocks required for downstream sterol and ubiquinone synthesis. Investigators characterizing peroxisomal disorders, lipid metabolism, or mevalonate pathway flux can apply the kit to cell culture supernatant and tissue lysate workflows. Unopened reagents should be stored refrigerated at 4 degrees C, while reconstituted standard aliquots remain stable through repeated freeze-thaw cycles when handled promptly.

The assay uses colorimetric readout on a standard microplate reader and ships with pre-coated wells, detection antibody, calibrator standards, wash buffer, and substrate solution sufficient for a full plate. Researchers profiling biomarker expression in disease cohorts, validating IDI2 knockdown or overexpression in transfected cell lines, or comparing IDI1 and IDI2 contributions across tissues will find the workflow compatible with routine ELISA procedures.