Human IDH3A ELISA Kit (HUFI03861)

Mitochondrial isocitrate dehydrogenase 3 (NAD+) catalytic subunit alpha (IDH3A) occupies a rate-controlling step of the tricarboxylic acid cycle, where the enzyme catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate with concomitant reduction of NAD+ to NADH. Researchers use the Assay Genie Human IDH3A ELISA Kit to quantify levels of this catalytic subunit in clinical research specimens, supporting studies of cellular bioenergetics, mitochondrial NADH production, and tumor metabolism where altered TCA cycle enzyme abundance has been linked to oncogenic reprogramming in multiple cancer models.

The kit is configured for in vitro research use only and is intended for laboratory workflows that require a quantitative immunoassay readout at a standard microplate reader. Components should be refrigerated on arrival, protected from light where indicated, and the working standard curve prepared fresh before each run; repeated freeze-thaw of diluted standards should be avoided. Each pack contains the reagents needed to process a full microplate of human clinical specimens, with protocols validated for measurement in blood-derived research samples, including biomarker screening panels that examine TCA cycle enzyme abundance relative to disease state or treatment response.