Human IAH1 ELISA Kit (HUFI03856)

Developed by Assay Genie for laboratory research, this ELISA kit enables quantitative measurement of human IAH1, a mitochondrial short-chain esterase involved in branched-chain amino acid catabolism and isoleucine degradation pathways. The kit supports studies exploring metabolic reprogramming in cancer cells, where altered IAH1 activity has been documented in hepatocellular and colorectal tumor tissues. Each pack contains a pre-coated microplate, lyophilized standards supplied in sealed vials, biotinylated detection antibody, streptavidin-HRP concentrate, wash buffer, and substrate solution. Researchers apply this assay to investigate enzyme expression in cell culture models, subcellular fraction preparations, and tissue protein extracts. Results integrate cleanly with downstream proteomics, pathway mapping, and drug response characterization. After the run, return all reagent vials to refrigerated storage at 4 °C, aliquot concentrated components upon first use, and avoid excessive freeze-thaw cycles. The complete workflow is intended for research use only.