Human Hypoxia Up Regulated 1 (HYOU1) ELISA Kit

Quantitative measurement of the endoplasmic reticulum-resident chaperone HYOU1 (also called ORP150 or GRP170) is the focus of this immunoassay kit from Assay Genie. The protein is a stress-induced member of the HSP70 family that accumulates in cells exposed to low oxygen, making it a useful biomarker in cancer hypoxia studies, ischemic injury models, and investigations of misfolded-protein disease.

The kit is supplied as a complete set containing a pre-coated microplate, lyophilized reference standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer concentrate, and substrate. Researchers typically prepare samples by centrifugation at 1000 x g, aliquot the supernatant, and process them either fresh or after frozen storage, with the recommendation to limit freeze-thaw cycles to preserve epitope integrity.

Compatible with standard plate-reader workflows, each kit supports batch processing in research use only investigations of human cell culture supernatants, tissue homogenates, and matched serum or plasma specimens. The supplied reagents are intended for in vitro measurement of endogenous HYOU1 protein in translational research programs.