Human Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit

Researchers investigating purine metabolism, Lesch-Nyhan syndrome biology, or gene expression normalization workflows can quantify HPRT1 protein directly with the Assay Genie human HPRT1 ELISA Kit, an immunoassay that pairs a pre-coated microplate with biotinylated detection reagents and streptavidin-HRP readout chemistry.

The kit is supplied as a 96-assay microplate and ships with pre-coated strips, calibrated protein standards, wash buffer, substrate solution, and a detection antibody sufficient to run a complete standard curve alongside replicate unknowns on a single plate.

Validated for use with multiple human-derived sample matrices, including tissue homogenate and cell lysate preparations as well as common liquid clinical specimen types, the assay gives laboratories a reproducible quantitative tool that complements mRNA-based housekeeping gene analysis. Reagents should be stored refrigerated at 4 degrees C to preserve activity; allow all components to reach room temperature before opening the sealed pouch of unused strips.