Human Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) ELISA Kit

Quantitative measurement of human Hydroxymethylglutaryl Coenzyme A Synthase is delivered through this ELISA from Assay Genie. The enzyme occupies a critical position in cellular metabolism, catalyzing the condensation of acetyl-CoA with acetoacetyl-CoA to generate HMG-CoA, which feeds into both cholesterol biosynthesis via the mevalonate pathway and ketone body production during hepatic ketogenesis. Researchers investigating metabolic disease, hepatic biology, and lipid homeostasis frequently monitor HMGCS as a marker of pathway activity.

Supplied as a complete kit for 96 assays, the test is validated for application with tissue homogenate preparations and a range of human-derived sample matrices. Standard absorbance-based detection enables quantitation on conventional microplate readers found in most research laboratories. Unopened kits should be refrigerated to preserve reagent activity through the indicated expiration date.

Typical research applications include studies of fatty acid oxidation disorders, non-alcoholic fatty liver disease, diabetic ketoacidosis, and mitochondrial bioenergetics. The specificity for human HMGCS supports cross-disciplinary research from cell biology laboratories examining transcriptional regulation of metabolic enzymes to clinical research programs studying ketone body metabolism.