Human Huntingtin Associated Protein 1 (HAP1) ELISA Kit

Huntingtin Associated Protein 1 (HAP1) is a cytoplasmic binding partner of the huntingtin protein, first identified through yeast two-hybrid screens and implicated in vesicular trafficking along microtubules and in receptor endocytosis within neurons. This quantitative immunoassay from Assay Genie is configured as a 96 assay kit and is intended for research use only, supporting investigations into Huntington's disease biology and related neurodegenerative pathways where HAP1 expression and isoform distribution (HAP1-A and HAP1-B) are studied in human samples. The kit accepts tissue homogenates, allowing laboratories to measure HAP1 levels in post-mortem brain tissue, cultured cell lysates, and similar preparation types relevant to studies of mutant huntingtin interactions, axonal transport, and autophagy. Components are supplied refrigerated on wet or dry ice, and the absorbance readout is captured on a standard microplate reader, making the workflow compatible with laboratories already running western blot, immunohistochemistry, or cell culture based follow-up experiments on HAP1 positive neurons. By providing a reproducible means to quantify endogenous HAP1 across experimental conditions, the kit aids researchers exploring how altered HAP1 abundance contributes to disease phenotypes, including studies pairing HAP1 measurement with mutant huntingtin expression in cell culture models.