Human HTRA2 (Serine protease HTRA2, mitochondrial) ELISA Kit (HUFI03876)

Assay Genie supplies this sandwich ELISA kit configured for the quantitative detection of human HTRA2 (Serine protease HTRA2, mitochondrial). The double-antibody sandwich format is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, with colorimetric readout based on TMB substrate and absorbance measurement at 450 nm.

The assay has a reported sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL, and is intended for storage at 2–8°C for 12 months. HTRA2 is described as a serine protease involved in mitochondrial function, apoptosis regulation, and cellular stress response, with dysregulation linked to Parkinson's disease, cancer, and neurodegenerative disorders. Associated identifiers include UniProt O43464 and synonyms such as HtrA2, OMI, Omi stress-regulated endoprotease, PRSS25, Serine protease 25, Serine proteinase OMI, and High temperature requirement protein A2.

Reported linearity across 1:2, 1:4, and 1:8 dilutions: Serum (n=5) 88–104%, 86–99%, 89–103%; EDTA Plasma (n=5) 89–100%, 85–91%, 84–94%; Heparin Plasma (n=5) 82–97%, 82–95%, 83–96%. Reported recovery ranges are Serum (n=5) 87–97% with average 91%, EDTA Plasma (n=5) 85–104% with average 95%, and Heparin Plasma (n=5) 87–93% with average 91%.